The method provides unprecedented high-resolution (a few tens of nm) imaging of the mechanical features of soft biological samples ( e.g. The latter corresponds to an AFM probe that periodically hits the sample surface while the pico-newton level interaction force is recorded from cantilever deflection. The instrumental progress makes it currently possible to generate a large amount of data in a relatively short time, which is particularly true for AFM operating in so-called PeakForce tapping mode (Bruker corporation). In ver5, the edges of the ROI box can be dragged individually.Atomic Force Microscopy (AFM) is a powerful technique for the measurement of mechanical properties of individual cells in two ( x × y) or three ( x × y × time) dimensions. This makes it nearly impossible to draw an ROI box that runs the full width or height of the imiage. Is there any version of NanoScope Analysis where the additional digits I need can be displayed?Ģ) In NA and NS ver6, section and step height analysis region of interest boxes are drawn using the Rotating box object, which has the annoying property that it can be expanded only from its center. However, in offline analysis in version 5 Nanoscope software, the step height analysis displays height with up to 5 significant figures. The above is true for offline analysis in version 6 software and in NanoScope Analysis thru version 1.5, as far as I know. In section analysis, the height difference is based on single point values. In step height analysis, the elevation of the upper and lower levels is based on an average of a selected range of data points.
This difference in display precision is ironic, because the step height value deserves more precision, not less. In step height analysis, the same data gives a displayed step height of 200 nm, with no digits after the decimal point. In section analysis, the vertical difference between two points is reported with 3 digits after the decimal point. This message concerns features that are inferior in NanoScope Analysis when compared with a benchmark software, NanoScope ver 5.31r1.ġ) In NanoScope Analysis, Section analysis provides a high precision measurement but Step Height analysis does not.
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